Whole genome sequencing of enteric organisms has enhanced the ability of public health professionals to detect, investigate, and confirm outbreaks. Understanding how sequence data is generated, analyzed, and visualized makes interpreting sequence data more accessible to users outside the laboratory. This course will provide a high-level overview of the topic, taking learners through the sequencing process from test tube to phylogenetic trees, with a focus on why this data matters to foodborne outbreak investigators.
Featured Speaker
Krisandra Allen, MPH, MB(ASCP)CM
Molecular Epidemiologist
Washington State Department of Health
Learning Objectives
- Describe two reasons why sequencing replaced PFGE as the dominant method of molecular subtyping of enteric pathogens
- Identify the two main analysis methods for WGS data of enteric pathogens
- Label the following parts of a phylogenetic tree: node, leaf, clade